The Gunners were paired with the Catalan giants when the draw for the first knockout round was made at UEFA headquarters in Nyon on Friday.
Arsenal were knocked out of last season's competition by Barcelona. They drew 2-2 at Emirates Stadium before losing 4-1 at the Nou Camp.
The teams also met in the 2006 Champions League Final where Barcelona came from behind to win 2-1 at the Stade de France.
The dates for our two-legged tie with Barcelona are as follows:
First leg
Arsenal v Barcelona
Wednesday 16th February 2011
Kick Off: 19.45 UK
To be played at Emirates Stadium
Second leg
Barcelona v Arsenal
Tuesday 8th March 2011
Kick Off: 19:45 UK
To be played at Camp Nou
